Airway and alveolar epithelial cells in culture
نویسندگان
چکیده
منابع مشابه
Growth and differentiation of conducting airway epithelial cells in culture.
The development of routine techniques for the isolation and in vitro maintenance of conducting airway epithelial cells in a differentiated state provides an ideal model to study the factors involved in the regulation of the expression of mucociliary differentiation. Several key factors and conditions have been identified. These factors and conditions include the use of biphasic culture techniqu...
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With the use of magnetic twisting cytometry, we characterized the mechanical properties of rat type II alveolar epithelial (ATII) cells in primary culture and examined whether the cells' state of differentiation and the application of deforming stresses influence their resistance to shape change. Cells were harvested from rat lungs as previously described (Dobbs LG. Am J Physiol Lung Cell Mol P...
متن کاملHuman alveolar epithelial type II cells in primary culture
Alveolar epithelial type II (AEII) cells are a key structure and defender in the lung but also are the targets in many lung diseases, including acute respiratory distress syndrome, ventilator-induced lung injury, and pulmonary fibrosis. We sought to establish an optimized method for high yielding and long maintenance of characteristics of primary human AEII cells to facilitate the investigation...
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Methods The experimental design was approved by the Ethics Committee of Federal University of Ouro Preto (UFOP)No.092/2012. Groups of female BALB/c mice (8 weeks old; 24.53±0.31g) were divided randomly in to five experimental groups as follows: Control group (CG) remained to air room; PBS group received aluminum hydroxide in phosphate buffered saline (PBS); OVA group (OVA) received ovalbumin an...
متن کاملMolecular diversity and function of K+ channels in airway and alveolar epithelial cells.
Multiple K(+) channels are expressed in the respiratory epithelium lining airways and alveoli. Of the three main classes [1) voltage-dependent or Ca(2+)-activated, 6-transmembrane domains (TMD), 2) 2-pores 4-TMD, and 3) inward-rectified 2-TMD K(+) channels], almost 40 different transcripts have already been detected in the lung. The physiological and functional significance of this high molecul...
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ژورنال
عنوان ژورنال: European Respiratory Journal
سال: 2019
ISSN: 0903-1936,1399-3003
DOI: 10.1183/13993003.00742-2019